DE Suggested Assays

VitaCyte relies on several biochemical tools to characterize and ensure the consistency of components used to prepare DE Collagenase. The Collagenase activity is characterized using both the traditional W√ľnsch (Pz-peptide)1 substrate and a more recently developed fluorescent microplate collagen degrading activity (CDA) assays. The CDA relies on fluorecein isothiocyanate (FITC) labeled calf skin collagen fibrils as substrate2. The B. polymyxa neutral protease activity (NPA) is measured using a FITC labeled BSA substrate3.