VitaCyteUnravelling Cell Isolation
VitaCyte manufactures and supplies GMP Grade purified-defined Clostridium histolyticum collagenase and protease enzymes to researchers, commercial cell suppliers, and institutions that manufacture cells as therapeutic agents. VitaCyte’s capabilities to custom manufacture products enables collaboration with companies who use enzymes for commercial applications.
Research
Cell Suppliers
Cell Therapy
Our ProductsPurified Cell and Tissue Dissociation Enzymes
Highly Purified
Lot to Lot Consistent
Best Performing Enzymes
Ability to Design Mixtures for Specific Applications
Ability to Customize Product to Meet Your Needs
Purified-Defined EnzymesWhat does it mean?
Collagenase suppliers can be divided into three categories:
Traditional Crude or Enriched Collagenase
Purified Collagenase
Purified-Defined Collagenase
Our StoryHow VitaCyte Began
VitaCyte’s co-founders, Bob McCarthy and Francis Dwulet, were key contributors to the development of the first commercial, purified collagenase-protease enzyme mixture used to prepare cells for subsequent transplantation. This improved tissue dissociation enzyme reagent contributed to success of the “Edmonton Protocol,” the first report of successful treatment of adult patients with Type 1 Diabetes where 7 of 7 patients were “insulin independent” one year after islet transplantation. Prior to the introduction of this purified enzyme reagent, most laboratories performing this clinical research procedure were spending significant amounts of time qualifying collagenase lots for their effectiveness to isolate human islets. Once a lot was selected, the lot qualification procedure was repeated within a year, often because of the instability of the enzymes on storage.
ResourcesAll things cell isolation
Isolation of Cells from Fat
Thursday, September 26, 2024 | 11 AM ET Presented by Stuart K. Williams, PhD In March 1964, Martin Rodbell reported that rat adipocytes isolated by digesting minced parametrial fat (fat around the uterus) using collagenase responded to externally delivered hormones....
Your Second Source of Cell Isolation Enzymes
case study Situation Cell isolation from tissue or recovery after in vitro cell culture often requires collagenase or protease enzymes. The enzyme-mediated steps of cell isolation or recovery methods are critical process parameters of the therapeutic cell...
An Update to a Widely Read Rodent Islet Isolation Methods Paper
Robert McCarthy Recently, I received an email from Research Gate that notified me as a co-author of a Jove 2012 publication, “Mouse islet of Langerhans isolation using a combination of purified collagenase and neutral protease,” that the article was read > 1500...
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For questions regarding enzyme useage, regardless if they are directly related to VitaCyte’s products, Customer Service is excellent. People at the company are eager to share their valuable experience and opinions on how best to perform procedures from donors of various species.
Latest NewsAll things cell isolation
ISCT 2024
We are proud to sponsor International Society for Cell & Gene Therapy 2024.May 28 – June 1, 2024 | Vancouver, CanadaAttendingRobert McCarthyPresident & Co-FounderAaron CraigDirector of Quality AssuranceAndrew BreiteAssociate Dir of QA & Customer Service...
DE Collagenase is Now PD Collagenase
The DE Collagenase product line will be discontinued and replaced with two Purified Define (PD) Collagenase formulation products, PD Collagenase 100 (Cat # 011-3010) and PD Collagenase 800 (Cat # 011-3020). The switch will take effect March 1, 2024. The new PD...
Meet Aaron Craig, the New Director of Quality Assurance
Aaron Craig is the new Director of Quality Assurance at VitaCyte, effective January 8, 2024. Aaron has over 20 years of experience managing and leading Eli Lilly’s, CBRE Workplace Solutions’, and MilliporeSigma’s quality assurance departments. Aaron’s expertise...