Join VitaCyte at The International Pancreas and Islet Transplant Association (IPITA), the International Xenotransplantation Association (IXA), and the Cell Transplant and Regenerative Medicine Society (CTRMS), 2023 Joint Congress this fall. October 26 – 29, 2023 | San Diego, CA, USA Sponsored Symposia VitaCyte is sponsoring a symposium at the joint congress. Join us in this
The development of the Liberase™ HI Purified Enzyme Blend product by an R&D team that included VitaCyte’s (VC’s) co-founders led to the company’s keen interest in optimizing the enzyme composition and dose used for human islet isolation. Several years ago, VC published a technical poster summarizing laboratory results that compared the performance of commercial collagenase-protease
Occam’s razor is a useful mental model to develop hypotheses to untangle complex problems. It can be summarized as follows: “Other things being equal, simpler explanations are generally better than more complex ones.” In 2011, McCarthy et al. proposed a hypothetical model of tissue dissociation that focused on how the synergistic interaction of collagenase and
In 1975, Godo Shusei manufactured a purified bacterial protease from Bacillus polymyxa (now reclassified and renamed as Paneibacillus polymyxa), trademarked Dispase™, to use in place of bovine or porcine trypsin for harvesting cells from culture.(1) Dispase is an animal-origin-free (AOF – no animal components used in fermentation or during purification process) protease less aggressive than
Astronomers search for life on other planets coined the phrase the “Goldilocks Zone” to describe the ideal conditions that will support life. The “life supporting” planet cannot be too close or too far away from the sun of its solar system. It must have temperate zones that allows liquid water to exist and have an
The selection and dose of neutral protease used for cell isolation is the critical factor that controls the outcome from a cell isolation procedure if and only if there is an excess of collagenase collagen degrading activity in the enzyme mixture. The primary function of collagenase is to strip a sufficient number of tropocollagen molecules
The original Liberase™ Purified Enzyme Blend product line was developed, manufactured, and sold in the U.S. in December 1994, by Boehringer Mannheim Biochemicals in Indianapolis. In 1995, the Liberase product was sold worldwide. The acquisition of Boehringer Manheim by Roche in 1998 and the subsequent transfer of the Liberase manufacturing to Germany in 2007, led
A primary concern for any operations or quality assurance manager who oversees a commercial or clinical cell manufacturing process is to tightly control the reagents used to perform critical process parameter steps. Clostridial histolyticum collagenases demand this oversight since the quality and consistency of these enzymes directly impact the reproducibility of cell isolation/recovery methods. However, with only
“Control” is the watchword commonly used to describe a robust manufacturing process. The Clostridium histolyticum collagenase enzymes used for cell isolation are critical process parameters for cell manufacturing. Control of these enzymes can be challenging because their consistency depends on the manufacturing process used to generate these products. Crude collagenase products were first sold in
The new year is here. Plan 2023 with a VitaCyte 3 month view calendar. Plan for success this year. Put it in your lab, office, kitchen, man cave, she shed. Email firstname.lastname@example.org or fill out the form below to get a calendar! Shipping Address USA only
Let’s compare traditional collagenase to VitaCyte’s collagenase products concerning three factors: Contact us to learn how we can optimize your cell isolation process.
An Environmentally Friendly Dry Ice Alternative We are no longer shipping with Dry Ice. Starting Feb. 1, 2022 we are shipping cold packages with our new Enviro Ice cold packs that are biodegradable and recyclable. Why the Change? Pandemic triggered supply disruptions have put significant pressures on couriers to deliver packages in the target timeline.
DIY Halloween Jack o’ Lantern Science Project Whether you’re making a spooky Halloween decoration or doing a fun science experiment with the family, this DIY smoking pumpkin will surely do the trick (or treat). Items you’ll need: How to make a smoking pumpkin: Send us a photo or video of your smoking pumpkins through social
Shop DE Collagenase Defined Enriched Collagenase
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Bring in the new year by meeting us at World Stem Cell Summit 2020. World Stem Cell Summit 2020 January 21-24, 2020 Hyatt Regency Miami400 South East Second Avenue Miami, Florida, 33131 Come visit Bob McCarthy and Andrew Breite from VitaCyte at booth #: TH18 in the Technology of Tomorrow area found in Riverfront Hall on
High Cost of Purified Collagenase Clostridium histolyticum collagenase is a critical reagent for isolating islets from human pancreatic tissue. The development of Liberase HI Purified Enzyme Blend overcame the problem of repetitive evaluation of different lots of crude collagenase to identify an acceptable product. C. histolyticum class I (C1) and class II (C2) collagenases and
A common approach for adopting or maintaining a consistent cell isolation procedure is to qualify lots of traditional (i.e., crude or enriched) collagenase products prior to use. This is a standard practice for most laboratories since it is an effective approach to help identify a lot of collagenase that works acceptably for the user’s cell
Crude Collagenase vs. DE Collagenase Price of DE Collagenase is comparable to equivalent products manufactured by Worthington or Sigma. % Collagenase + Protease is the mass of bacterial enzymes (collagenase + protease) as a percentage of bacterial protein in the product. Crude collagenase is a lyophilized Clostridium histolyticum culture supernatant, all the protein in product is
Do you feel like Bill Murray re-living Groundhog Day when you qualify new lots of traditional collagenase to isolate rodent islets? Break out of the unproductive time loop by switching to CIzyme RI, a purified, defined enzyme mixture designed for rodent islet isolation. Compare your rodent islet yields with those obtained using CIzyme RI by