A primary concern for any operations or quality assurance manager who oversees a commercial or clinical cell manufacturing process is to tightly control the reagents used to perform critical process parameter steps. Clostridial histolyticum collagenases demand this oversight since the quality and consistency of these enzymes directly impact the reproducibility of cell isolation/recovery methods. However, with only
Are Your Cell Isolation Enzymes Under Control?
“Control” is the watchword commonly used to describe a robust manufacturing process. The Clostridium histolyticum collagenase enzymes used for cell isolation are critical process parameters for cell manufacturing. Control of these enzymes can be challenging because their consistency depends on the manufacturing process used to generate these products. Crude collagenase products were first sold in
The new year is here. Plan 2023 with a VitaCyte 3 month view calendar. Plan for success this year. Put it in your lab, office, kitchen, man cave, she shed. Email firstname.lastname@example.org or fill out the form below to get a calendar! Shipping Address
Traditional vs VitaCyte Collagenase
Let’s compare traditional collagenase to VitaCyte’s collagenase products concerning three factors: Purity Lot Qualification Flexibility Contact us to learn how we can optimize your cell isolation process.
VitaCyte is Switching to Enviro Ice
An Environmentally Friendly Dry Ice Alternative We are no longer shipping with Dry Ice. Starting Feb. 1, 2022 we are shipping cold packages with our new Enviro Ice cold packs that are biodegradable and recyclable. Why the Change? Pandemic triggered supply disruptions have put significant pressures on couriers to deliver packages in the target timeline.
How to Make a Smoking Pumpkin
DIY Halloween Jack o’ Lantern Science Project Whether you’re making a spooky Halloween decoration or doing a fun science experiment with the family, this DIY smoking pumpkin will surely do the trick (or treat). Items you’ll need: Carved Pumpkin Dry Ice Hot Water Light Halloween Decorations (Optional) How to make a smoking pumpkin: Carve your
Crude Collagenase vs. DE Collagenase
Shop DE Collagenase Defined Enriched Collagenase
Customer Satisfaction Results
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World Stem Cell Summit 2020
Bring in the new year by meeting us at World Stem Cell Summit 2020. World Stem Cell Summit 2020 January 21-24, 2020 Hyatt Regency Miami400 South East Second Avenue Miami, Florida, 33131 Come visit Bob McCarthy and Andrew Breite from VitaCyte at booth #: TH18 in the Technology of Tomorrow area found in Riverfront Hall on
Reducing the Cost of Human Islet Isolation
High Cost of Purified Collagenase Clostridium histolyticum collagenase is a critical reagent for isolating islets from human pancreatic tissue. The development of Liberase HI Purified Enzyme Blend overcame the problem of repetitive evaluation of different lots of crude collagenase to identify an acceptable product. C. histolyticum class I (C1) and class II (C2) collagenases and
Lot Qualification: A Common but Costly Pathway to Select Collagenase Products for Cell Isolation
A common approach for adopting or maintaining a consistent cell isolation procedure is to qualify lots of traditional (i.e., crude or enriched) collagenase products prior to use. This is a standard practice for most laboratories since it is an effective approach to help identify a lot of collagenase that works acceptably for the user’s cell
Overcoming the Limitations of Traditional Collagenase Products
Traditional collagenase products (i.e., crude or enriched collagenase) have been used for over 50 years to isolate cells from tissue. Little has changed in the selection and use of these products since the original introduction of crude collagenase in the early 1960’s for use in cell isolation procedures. Many laboratories pre-qualify lots prior to purchase
Which One Are You Using?
Crude Collagenase vs. DE Collagenase Price of DE Collagenase is comparable to equivalent products manufactured by Worthington or Sigma. % Collagenase + Protease is the mass of bacterial enzymes (collagenase + protease) as a percentage of bacterial protein in the product. Crude collagenase is a lyophilized Clostridium histolyticum culture supernatant, all the protein in product is
Tired of Re-living Groundhog Day?
Do you feel like Bill Murray re-living Groundhog Day when you qualify new lots of traditional collagenase to isolate rodent islets? Break out of the unproductive time loop by switching to CIzyme RI, a purified, defined enzyme mixture designed for rodent islet isolation. Compare your rodent islet yields with those obtained using CIzyme RI by
New Paradigm for Collagenase Products
Essential Information on Enzyme Activities Required for Effective Cell Isolation Part A: Overview of Current Knowledge and State of the Art Overview Worthington Biochemicals began supplying Clostridium histolyticum collagenase for cell isolation in the early 1960s. Worthington’s Tissue Dissociation Guide summarizes the use of these enzymes for cell isolation. They categorized their collagenase products into
VitaCyte to Exhibit at the 9th EPITA Symposium/38th AIDPIT Workshop
VitaCyte and its European distributor, PeloBiotech, will exhibit at the Workshop held 27 to 29 January 2019 at Congresspark Igls-Innsbruck, Austria. VitaCyte’s broad line of enriched/purified collagenase and purified neutral protease products are rigorously characterized to ensure lot to lot consistency. Collagenase Gold is a low cost product that performs similarly to the higher priced
Evolution of Enzyme Requirements for Human Islet Isolation, Link to a Recent Review
The quality of collagenase used for mammalian islet isolation has been a focus of clinical research scientists soon after Ballinger and Lacy demonstrated that islet transplantation could correct chemically induced diabetes in inbred strains of rats (1). Clostridium histolyticum collagenase became a critical reagent because of the need to isolate sufficient numbers of functional islets
Truth vs. Fiction: Thermolysin & Human Islet Isolation
More human islet isolations have been performed using purified collagenase and thermolysin than any other purified enzyme mixture (1). Several reports have shown the adverse effects when using thermolysin for human islet isolation (2, 3). This blog post reviews the truth and fiction when using thermolysin for human islet isolation. It will also briefly discuss
New Insights for Using Purified Enzymes in Human Islet Isolation
The introduction of Liberase™ HI Purified Enzyme Blend in 1994 to improve the consistency of enzymes used to recover human pancreatic islets for subsequent transplantation contributed to the growth of clinical islet transplantation.(1) Liberase™ HI set a benchmark for comparing the performance of all subsequent products used for this application.(2, 3) A series of reports
Variability in Islet Yields May be Due to the Molecular Form of Class 1 Collagenase
The transition of enzyme suppliers in 2007 from Liberase™ HI to Nordmark/Serva’s NB-1 collagenase & NB protease for isolation of human islets used in Collaborative Islet Transplantation consortium clinical trial was problematic for some expert islet labs but not for others. During this period, the number of allo-islet transplantations performed in North America was at