Simple approach to optimize collagenase enzyme mixtures to improve cell recovery Current state of the art No simple method exists for defining optimal collagenase enzyme formulations for the recovery of primary cells from tissue. The unknown biochemical composition of traditional crude or enriched collagenase products, combined with variability in enzyme activities between each lot ofRead more about Simple approach to optimize collagenase enzyme mixtures to improve cell recovery[…]
Setting a new paradigm for low cost collagenase The current paradigm for collagenase: Crude collagenase is impure, as reflected by the brown to black color of the product. Biochemical heterogeneity between different lots of crude collagenase is due to impurities and is reflected by inconsistent results after use in cell isolation procedures. Collagenase products areRead more about Setting a new paradigm for low cost collagenase[…]
Does collagenase truly deserve top billing as the key enzyme for isolating primary cells? Collagenase quality For many years, experts in cell isolation have stated the importance of collagenase quality for successful cell isolation.1,2 What does collagenase quality mean? Many investigators have found it difficult to correlate the enzymatic analysis data summarized on a CertificateRead more about Does collagenase truly deserve top billing as the key enzyme for isolating primary cells?[…]
Over the past several years, you may have found it difficult to find a good lot of collagenase from suppliers. If you’ve not experienced this in the past, you may wonder why this is happening now. Based upon my experience in collagenase product manufacture, and my interest in how business practices change over time, IRead more about 3 Factors may explain why you cannot find a “good lot” of collagenase[…]
What causes variation in the color of traditional collagenase products? Traditional collagenase products can range in color from a light tan to dark brown. Color is directly influenced by the collagenase manufacturing process. Collagenases and neutral proteases are the key enzymes in these products principally responsible for degrading extracellular matrix and in turn, releasing cellsRead more about Collagenase color: what does it tell you about product quality?[…]
Most lyophilized collagenase containing tissue dissociation enzymes (TDEs) used today have a wide spectrum of color – tan to black – depending on the product. This color serves as a litmus test indicating that these enzymes are not pure and are likely have high variability in enzyme activity between lots. The information below presents more details of the biochemical characteristics of crude or enriched collagenase products and shows the benefits of using purified enzymes in place of these products.