Applied Uses for VitaCyte Products

Uses for VitaCyte’s products are constantly evolving as users in the field find interesting and innovative new ways to apply them to their research and clinical work.

We’ve developed several of our products with specific uses in mind, and the application notes on these pages reflect our knowledge of intended or suggested uses.

The information provided in this section is not comprehensive and does not (nor can it, feasibly) account for variations in the source of the tissue, tissue quality, lab protocols, product handling, or a myriad of other influencing factors. We attempt, instead, to provide a high-level, baseline approach — a starting point — for using our products.

We welcome opportunities to collaborate on new uses or approaches to using our products. Please contact us.

Overviews and Methods

Islets

Human Islet Overview. Overview discussion of common protocols used in human islet isolation. Includes a table listing factors to consider when focusing on enzymes used in these isolation procedures.

Rodent Islet Overview. Overview discussion of common protocols used in rodent islet isolations. Includes a link to a white paper that summarizes a survey of the affect of rodent strain, age, sex, or nutritional state on expected islet yields when purified enzymes were used to isolate islets.

Adipose Tissue

Human Adipose Stromal Vascular Fraction Cells. Overview discussion of historical approaches to isolating adiopose human stromal vascular fraction cells.

Hepatocytes

Rodent Hepatocytes Overview. Overview discussion of methods for isolating rodent hepatocytes. Includes a table of recommended products and doses for use with rat and mouse islet isolations.

Other Cells

Various Cells and SpeciesOverview of potential uses for VitaCyte’s unique DE Collagenase product line, across various cell types and species. Includes a reference table to translate the dose of crude of enriched collagenase from other manufacturers to the type of DE Collagenase and dose to use for your specific application.