Recommended products: Defined Enriched Collagenases
- DE Collagenase 10/100
- DE Collagenase 20/200
- DE Collagenase 40/400
- DE Collagenase 60/600
- DE Collagenase 80/800
Use our Defined Enriched (DE) Collagenase products as replacements for the crude, enriched, or purified collagenase products listed in the table below.
Table 1: Recommended Products
Product Name & Description
For every mg of collagenase product use following mg of DE Collagenase ± BP Protease products
|Type 1: contains average amounts of assayed activities||DE 10 or 100||1|
|Type 2: contains greater clostripain activity than Type 1||DE 10 or 100||1|
|Type 3: contains lower protease activity than type 1||DE 20 or 200||0.65|
|Type 4 contains lower protease and tryptic activity (clostripain) than Type 1||DE 20 or 200||0.65|
|Type V contains higher collagenase and protease activity||DE 10 or 100||1|
|Type I: similar to Worthington Type I but with higher collagenase activity||DE 10 or 100||1|
|Type IA: similar to Worthington Type I but with higher collagenase activity||DE 10 or 100||1|
|Type II: similar to Worthington Type II but with higher collagenase activity||DE 20 or 200||0.65|
|Type III: purified collagenase with trace amounts of protease||Collagenase Gold||1|
|Type IV: lot tested for release of rat hepatocytes||DE 10 or 100
|Follow protocol for rodent hepatocytes|
|Type V: lot tested for release of rat islets||DE 40 or 400||Follow protocol for rodent islets|
|Type VII: purified collagenase substantially free of protease and clostripain||Collagenase Gold||Contact VitaCyte for recommendation|
|Type VIII: tested for release of rat epididymal adipocytes or hepatoctyes||DE 10 or 100||1
Follow protocol for rodent hepatocytes
|Type XI enriched collagenase prepared for islet isolation||DE 60 or 600||1|
|Manufactured by Roche; distributed by Sigma|
|Collagenase P||DE 20 or 200||1|
|Liberase TL Research Grade||DE 40 or 400||1|
|Liberase TM Research Grade||DE 10 or 100||2|
|Liberase TH Research Grade||DE 100||Contact VitaCyte for recommendation|
|Liberase DL Research Grade||DE 80 or 800||1|
|Liberase DH Research Grade||DE 20 or 200||1|
Note: Sigma Type IV, V, and VII are function tested lots of crude collagenase for isolation of the stated cell types. In these cases, you are referred to use of the protocols for these cells that can be found in Applied Use.
Recommended method: Varies
Recommended method is dependent on the user’s experience.
Expected cell yield
Expected results are dependent on the user’s experience.
Crude collagenase has been used for many years to isolate a wide variety of cells. The major disadvantage for using recommendations provided by other manufacturer’s or results published in the scientific literature is that cell function or yields are dependent on the unique characteristics of the lot of crude collagenase used in the isolation procedure. The results obtained by one investigator may not be the same as those obtained by another because of the lot to lot variability of crude collagenase. All crude collagenase products are minimally purified, C. histolyticum culture supernatants whose composition reflects the biochemical composition at the end of the fermentation process.
VitaCyte’s low cost, DE Collagenase is designed to overcome many of the limitations of traditional collagenase products (i.e., crude or enriched collagenase) as shown in the table below.
Table 2: Comparison of Traditional Collagenase and VitaCyte DE Collagenase Products
|Need for lot pre-qualification||
Only on first use of product
|Degree of experimental control||
|Translation of protocol to clinical use||
Resources & Reviews
For more information on any of these issues, request VitaCyte’s white paper on “Lot Qualification: A common but costly pathway to select enzymes for cell isolation” or a 33 min video of a tech talk by Bob McCarthy presented at the 2016 American Society of Cell Biology meeting: “Collagenase: Moving from black box to transparent knowledge”. The video reviews basic aspects of collagenase as it applies to cell isolation and presents an overview of VitaCyte’s DE Collagenase product line.