by Jessica Helweg | Sep 16, 2019
Temperatures in the range of 35-37°C will result in maximum activity of collagenase degradation of native collagen. A 5°C reduction in temperature (30°C) will result in an approximate 60% loss of maximal activity for class 1 and class 2 collagenase. In contrast, the...
by Jessica Helweg | Sep 16, 2019
Yes. VitaCyte’s collagenase products are chromatographically purified to minimize contamination of protease. Collagenase with negligible protease contamination ensures minimal loss of collagen degradation activity during storage or as a frozen solution. Purified...
by Jessica Helweg | Sep 16, 2019
No. VitaCyte’s purified collagenases and neutral proteases, as well as our application specific formulations, are all aseptically dispensed in a biological safety cabinet located within a controlled environment. To ensure sterility, we recommend the user filter the...
by Jessica Helweg | Sep 16, 2019
These results cannot be compared since different substrates and assay principles are used to measure this activity. VitaCyte’s kinetic fluorescent microplate CDA assay detects an increase in fluorescence as collagenase unravels and degrades fluorescein isothiocyanate...
by Jessica Helweg | Sep 16, 2019
CIzyme Collagenase HA (high activity) and MA (moderate activity) differ only in the amount of truncated class 1 (C1) collagenase present. Truncated C1 is defined as that form of C1 that has lost the carboxy terminal collagen binding domain. Truncated C1 has a mass...
by Jessica Helweg | Sep 16, 2019
Yes. Internal studies have shown the reconstituted collagenase is stable as a frozen solution between -15 to -25°C for at least 1 year as long as no other protease enzymes have been added to the collagenase solution. For ensured stability it is best to minimize the...
by Jessica Helweg | Sep 16, 2019
We recommend using sterile or RODI water for the initial reconstitution of the lyophilized protein. For details, refer to the products’ package insert. After dissolution, dilute the enzyme to the appropriate volume for use in your application. If you want to use a...
