What are the optimal temperatures for maximal collagenase and protease activity?

Temperatures in the range of 35-37°C will result in maximum activity of collagenase degradation of native collagen. A 5°C reduction in temperature (30°C) will result in an approximate 60% loss of maximal activity for class 1 and class 2 collagenase. In contrast, the neutral proteases BP Protease and thermolysin are less dramatically affected by temperature and maintain ≥ 80% their maximal activity at 26°C.