Guide to abbreviations used to label VitaCyte’s products or technical support literature.

Product abbreviations: Collagenase HA = purified collagenase with high collagen degradation activity (CDA) Collagenase MA = purified collagenase with moderate CDA (about 50% of Collagenase HA) BP Protease = neutral protease from Bacillus polymyxa (DispaseTM Equivalent enzyme) DE Collagenase = Defined Enriched collagenase CIzyme RI = application specific enzyme mixture for rodent islet isolation CIzyme

Why don’t you offer a formulation for my specific application?

There are over 200 mature cell types in mammals. A limited number of these cells are routinely used for research studies. These include islets and acinar cells from the pancreas, hepatocytes and non-parenchymal cells from the liver, cardiomyocytes, neural cells, and different types of kidney cells. For many of these cells, VitaCyte provides a product,

What are application specific formulations?

Application Specific Formulations are pre-blended mixtures of purified collagenase and protease in optimal ratios for isolation of cells from a particular tissue and/or species. These formulations provide a recommended dose for use with an associated method. These effectiveness of these product to isolate specific cell types was demonstrated by several different laboratories. Currently, VitaCyte offer

How do I transition from a Sigma, Worthington, or Nordmark collagenase product to a VitaCyte collagenase product?

This is dependent on the cell you are isolating. VitaCyte has enzyme product/formulations and recommended doses to isolate rodent islets, porcine islets, non-human primate islets, and human islets; rodent and human hepatocytes, and rodent cardiomyocytes, and human adipose derived stromal vascular fraction cells from human lipoaspirate. These recommendations can be found when you click on

Are your enzymes approved for clinical use?

To be determined. VitaCyte’s products are labeled for research use only or as GMP Grade. All products are manufactured in accordance with the principles for clinical trial material outlined in the International Conference on Harmonization (ICH) document ICH Q7A,  “Good Manufacturing Practice Guidance for Active Pharmaceutical Ingredients”. The GMP Grade products are bottled and lyophilized

What are the optimal temperatures for maximal collagenase and protease activity?

Temperatures in the range of 35-37°C will result in maximum activity of collagenase degradation of native collagen. A 5°C reduction in temperature (30°C) will result in an approximate 60% loss of maximal activity for class 1 and class 2 collagenase. In contrast, the neutral proteases BP Protease and thermolysin are less dramatically affected by temperature

Do I have to supplement the purified collagenase with a protease source?

Yes.  VitaCyte’s collagenase products are chromatographically purified to minimize contamination of protease. Collagenase with negligible protease contamination ensures minimal loss of collagen degradation activity during storage or as a frozen solution. Purified collagenase alone is ineffective in releasing cells from tissue since protease activity is required to degrade other extracellular matrix proteins (see Mechanism FAQs).

Are your enzymes sterile?

No. VitaCyte’s purified collagenases and neutral proteases, as well as our application specific formulations, are all aseptically dispensed in a biological safety cabinet located within a controlled environment. To ensure sterility, we recommend the user filter the enzyme solution through a 0.2 µm sterile filter immediately before use.

Why is your collagen degradation units per mg different than other vendors?

These results cannot be compared since different substrates and assay principles are used to measure this activity. VitaCyte’s kinetic fluorescent microplate CDA assay detects an increase in fluorescence as collagenase unravels and degrades fluorescein isothiocyanate conjugated to collagen fibrils. Measurements are made every 150 seconds. By contrast, the Mandl is an endpoint assay where collagenase

What buffer should I use to reconstitute the lyophilized enzyme?

We recommend using sterile or RODI water for the initial reconstitution of the lyophilized protein.  For details, refer to the products’ package insert. After dissolution, dilute the enzyme to the appropriate volume for use in your application. If you want to use a solution other than water to reconstitute the lyophilized protein, avoid using solutions