by Jessica Helweg | Sep 16, 2019
No. Poorly defined, traditional collagenase products contain between 3-8 and 10-20% (w/w) collagenase. Collagenase is usually degraded during the fermentation or material processing procedure. Recent studies have indicated that proteolytic degradation of collagenase...
by Jessica Helweg | Sep 16, 2019
Clostripain is a neutral protease secreted by C. histolyticum during the fermentation process. It is a tryptic like enzyme that is measured using the same peptide substrate to measure trypsin activity (N-benzoyl-L-arginine ethyl ester, BAEE). Clostripain is a...
by Jessica Helweg | Sep 16, 2019
No. Although each manufacturer may use similar methods, they will use different lots of substrate from different suppliers, make additional modifications to the assay procedure, as well as other changes, it is safe to assume there is poor correlation when comparing...
by Jessica Helweg | Sep 16, 2019
Collagenase activity can be assessed by a number of different enzyme activity assays. The enzyme activity is defined by the substrate so peptidase activity is detected when Pz-peptide (Wunsch assay) or FALGPA is used as substrates; gelatinase activity is detected...
