No. Poorly defined, traditional collagenase products contain between 3-8 and 10-20% (w/w) collagenase. Collagenase is usually degraded during the fermentation or material processing procedure. Recent studies have indicated that proteolytic degradation of collagenase leads to lower collagen degradation activity that translates into use of higher amounts of enzyme in the cell isolation procedure. There is
What does tryptic-like activity mean? How do you measure clostripain activity?
Clostripain is a neutral protease secreted by C. histolyticum during the fermentation process. It is a tryptic like enzyme that is measured using the same peptide substrate to measure trypsin activity (N-benzoyl-L-arginine ethyl ester, BAEE). Clostripain is a sulfhydrl enzyme that expresses maximal enzyme activity under reducing conditions (i.e., pre-incubation with 2 mercapatoethanol or other
Do all the collagenase manufacturers use similar assays so I can assume the activities between suppliers are similar?
No. Although each manufacturer may use similar methods, they will use different lots of substrate from different suppliers, make additional modifications to the assay procedure, as well as other changes, it is safe to assume there is poor correlation when comparing enzyme activity results from different suppliers.
What is the difference between the enzyme assays that are commonly included in a certificate of analysis?
Collagenase activity can be assessed by a number of different enzyme activity assays. The enzyme activity is defined by the substrate so peptidase activity is detected when Pz-peptide (Wunsch assay) or FALGPA is used as substrates; gelatinase activity is detected when gelatin or azocoll are as substrates; collagen degradation activity (CDA) is detected when using