Can I apply knowledge of this mechanism to traditional collagenase products?


It is very difficult to do for several reasons. First, the assays reported on the Certificate of Analysis do not provide sufficient detail to define the different functional forms of collagenase. Collagenase in crude or enriched collagenase products contain intact class I and class II, truncated class I collagenase and degraded forms of class I or class II collagenase that cannot degrade native collagenase  (non-functional forms). The Mandl assay is commonly used to assess collagen degradation activity in traditional collagenase products. Recently, it has been shown that this assay can be imprecise and the collagen degradation activity reflects the class or collagenase rather than its efficiency to degrade native collagen.