What is an acceptable rodent islet yield?
Isolation of rodent islets is a unique skill that usually requires a person interested in adopting this skill to receive advice or training from an islet isolation expert who will provide a demonstration of the method but will also share often unwritten recommendations that are often critical to the success of the procedure. A complication arises when either individual is faced with isolating islets from a new and different rodent strain. The key question asked is what islet yields should I expect? It has been known for many years that islet yields vary by the age, sex, and strain of the animal. These expected islet yields are rarely published in the scientific literature and if mentioned, they are briefly noted in the materials and methods or in a table in the results section. Commonly, the primary way an individual obtains this information is to ask their colleagues about their islet isolation experience using animals with similar characteristics: age, sex, and strain. To provide a resource to the rodent islet isolation community, VitaCyte surveyed its users and asked them to submit rodent islet yields isolated from different strains of mice and rats. These results are restricted to those isolations performed using purified collagenase and protease for reasons outlined in the white paper.
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