by Jessica Helweg | Sep 16, 2019
Maybe. It is very difficult to do for several reasons. First, the assays reported on the Certificate of Analysis do not provide sufficient detail to define the different functional forms of collagenase. Collagenase in crude or enriched collagenase products contain...
by Jessica Helweg | Sep 16, 2019
The success of cell isolation requires excess collagen degradation activity and selection of an appropriate neutral protease used at an optimal dose. This statement reflects the two assumptions of enzymatic tissue dissociation: Enriched or purified collagenase (with...
by Jessica Helweg | Sep 16, 2019
The Liberase projects showed that collagenase and neutral protease were the enzymes required for the release of islets from human pancreatic tissue. Liberase contains purified class I and class II C histolyticum collagenase, and thermolysin or Dispase. Different...
