Can I apply knowledge of this mechanism to use to traditional collagenase products?

Maybe. It is very difficult to do for several reasons. First, the assays reported on the Certificate of Analysis do not provide sufficient detail to define the different functional forms of collagenase. Collagenase in crude or enriched collagenase products contain intact class I and class II, truncated class I collagenase and degraded forms of class

What factors should be tightly controlled to ensure effective release of cells from tissue?

The success of cell isolation requires excess collagen degradation activity and selection of an appropriate neutral protease used at an optimal dose. This statement reflects the two assumptions of enzymatic tissue dissociation: Enriched or purified collagenase (with minimal contaminants) has a restricted specificity to degrade only native collagen or gelatin which are extracellular matrix proteins.

How does collagenase and protease release cells from tissues/organs?

The Liberase projects showed that collagenase and neutral protease were the enzymes required for release of islets from human pancreatic tissue. Liberase contains purified class I and class II C histolyticum collagenase, and thermolysin or Dispase. Different formulations and doses were used to isolate cells from different tissues. The identification of the C. histolyticum genes