by Jessica Helweg | Nov 10, 2016
The Dispase II product is a much cruder preparation of neutral protease than our BP protease, but both originate from Paenibacillus polymyxa fermentations . The FITC-BSA specific activity of the Dispase II product is less than 10% that of our BP Protease and can vary...
by Jessica Helweg | Nov 10, 2016
Thermolysin and BP Protease are entirely animal origin free. A porcine gelatin peptone is used during the fermentation step of natural collagenase production. While this animal sourced material is required to stimulate collagenase production from the biosynthesis from...
by Jessica Helweg | Nov 10, 2016
Yes. VitaCyte’s collagenase products are chromatographically purified. The steps utilized in the purification process remove the protease activity that is normally present in the crude and enriched collagenase products from other vendors. Since collagenases have a...
by Jessica Helweg | Nov 10, 2016
Yes. Selection of a protease in many cases is simply due to the historical precedence of the given cell isolation protocol being followed; however, that is not to say a different one would not work. The isolation of human islets is a perfect example in which...
by Jessica Helweg | Nov 10, 2016
Collagenase HA and MA differ only in the amount of truncated class 1 collagenase present. Both are 60%:40% blends of highly purified class 1 (C1) and class 2 collagenase (C2), respectively. The Collagenase MA will have a lower CDA specific activity relative to the HA...
