Knowledge is power to improve cell isolation
Thursday, November 21, 2024 | 11 AM ET
Presented by
Bob McCarthy
C. histolyticum collagenases have been used in the early 1960s for cell isolation, but most users have a limited understanding of how these enzymes dissociate tissue. The webinar will review an improved understanding of purified collagenase-protease enzyme mixtures by applying knowledge of collagenase structure-function. This knowledge led to the development of a practical model for understanding collagenase-protease-mediated tissue dissociation. The model led to several new insights to improve the use of collagenases in cell isolation.
-
- Insight 1: Why lot prequalification is required before using traditional collagenase products
- Insight 2: How purified enzymes led to the optimization of an enzyme formulation for rodent hepatocyte isolation
- Insight 3: Development of a rational design method for optimizing collagenase-protease enzyme mixtures
The webinar will conclude by discussing how this new knowledge aligns with the ISO 20399 Standard on Ancillary Materials.
Download Presentation Slides
Meet the Presenter
Robert McCarthy
VitaCyte
Bob McCarthy received a Ph.D. in immunology from Indiana University and did post-graduate training at the Mayo Clinic in Rochester, MN, on immunological research and development of clinical immunoassays. He joined Boehringer Mannheim Biochemicals in Indianapolis in 1987 as a Research Investigator and moved to Business Development and later Strategic Planning after the Roche acquisition of Boehringer Mannheim in 1998. In 1992, he led the R&D team that developed the Liberase HI Purified Enzyme Blend product to improve human islet recovery.
In 2004, Francis Dwulet and Bob co-founded VitaCyte to improve the manufacture and characterization of collagenase and protease enzymes used for cell isolation and other biomedical applications. VitaCyte’s mission is to develop products that increase research productivity or improve clinical outcomes.