Publications
Low Cost, Enriched Collagenase-Purified Protease Enzyme Mixtures Successfully Used for Human Islet Isolation
Loganathan G, Hughes MG, Szot GL, Smith KE, Hussain A, Collins DR, Green ML, Dwulet FE, Williams SK, Papas KK, McCarthy RC, Balamurugan AN. Low Cost, Enriched Collagenase-Purified Protease Enzyme Mixtures Successfully Used for Human Islet Isolation. OBM Transplantation 2019;3(2):14; doi:10.21926/obm.transplant.1902064.
Evolution of Enzyme Requirements for Human Islet Isolation
Improved Recovery of Human Islets From Young Donor Pancreases Utilizing Increased Protease Dose to Collagenase for Digesting Peri‐Islet Extracellular Matrix
Beneficial Effect of Recombinant rC1rC2 Collagenases on Human Islet Function: Efficacy of Low‐Dose Enzymes on Pancreas Digestion and Yield
Identifying Effective Enzyme Activity Targets for Recombinant Class I and Class II Collagenase for Successful Human Islet Isolation
N. Balamurugan, Appakalai & Green, Mike & Breite, Andrew & Loganathan, Gopalakrishnan & Wilhelm, Josh & Tweed, Ben & Vargova, Lenka & Lockridge, Amber & Kuriti, Manikya & Hughes, Jr, Michael & Williams, Stuart & J. Hering, Bernhard & E. Dwulet, Francis & Mccarthy, Robert. (2015). Identifying Effective Enzyme Activity Targets for Recombinant Class I and Class II Collagenase for Successful Human Islet Isolation. Transplantation Direct. 2. 1. 10.1097/TXD.0000000000000563.
Characterization and Functional Assessment of Clostridium Histolyticum Class I (C1) Collagenases and the Synergistic Degradation of Native Collagen in Enzyme Mixtures Containing Class II (C2) Collagenase
Successful Human Islet Isolation and Transplantation Indicating the Importance of Class 1 Collagenase and Collagen Degradation Activity Assay
Development and Application of Purified Tissue Dissociation Enzyme Mixtures for Human Hepatocyte Isolation
Effectiveness of Different Molecular Forms of C. histolyticum Class I Collagenase to Recover Islets
A New Enzyme Mixture to Increase the Yield and Transplant Rate of Autologous and Allogeneic Human Islet Products
Tissue Dissociation Enzymes for Isolating Human Islets for Transplantation: Factors to Consider in Setting Enzyme Acceptance Criteria
Tissue Dissociation Enzyme Neutral Protease Assessment
Development and Characterization of a Collagen Degradation Assay to Assess Purified Collagenase Used in Islet Isolation
Optimizing Porcine Islet Isolation to Markedly Reduce Enzyme Consumption Without Sacrificing Islet Yield or Function
Mouse Islet of Langerhans Isolation Using a Combination of Purified Collagenase and Neutral Protease