Low Cost, Enriched Collagenase-Purified Protease Enzyme Mixtures Successfully Used for Human Islet Isolation
Evolution of Enzyme Requirements for Human Islet Isolation
McCarthy RC, Green ML, Dwulet FE. Evolution of Enzyme Requirements for Human Islet Isolation. OBM Transplantation 2018;2(4):024; doi:10.21926/obm.transplant.1804024.
Improved Recovery of Human Islets From Young Donor Pancreases Utilizing Increased Protease Dose to Collagenase for Digesting Peri‐Islet Extracellular Matrix
Loganathan G, Subhashree V, Narayanan S, et al. Improved recovery of human islets from young donor pancreases utilizing increased protease dose to collagenase for digesting peri‐islet extracellular matrix. Am J Transplant. 2018;00:1–13. https://doi.org/10.1111/ajt.15111.
Beneficial Effect of Recombinant rC1rC2 Collagenases on Human Islet Function: Efficacy of Low‐Dose Enzymes on Pancreas Digestion and Yield
Loganathan G, Subhashree V, Breite AG, et al. Beneficial effect of recombinant rC1rC2 collagenases on human islet function: Efficacy of low‐dose enzymes on pancreas digestion and yield. Am J Transplant. 2018;18:478–485. https://doi.org/10.1111/ajt.14542.
Identifying Effective Enzyme Activity Targets for Recombinant Class I and Class II Collagenase for Successful Human Islet Isolation
N. Balamurugan, Appakalai & Green, Mike & Breite, Andrew & Loganathan, Gopalakrishnan & Wilhelm, Josh & Tweed, Ben & Vargova, Lenka & Lockridge, Amber & Kuriti, Manikya & Hughes, Jr, Michael & Williams, Stuart & J. Hering, Bernhard & E. Dwulet, Francis & Mccarthy, Robert. (2015). Identifying Effective Enzyme Activity Targets for Recombinant Class I and Class II Collagenase for Successful Human Islet Isolation. Transplantation Direct. 2. 1. 10.1097/TXD.0000000000000563.
Characterization and Functional Assessment of Clostridium Histolyticum Class I (C1) Collagenases and the Synergistic Degradation of Native Collagen in Enzyme Mixtures Containing Class II (C2) Collagenase
A.G. Breite, R.C. McCarthy, F.E. Dwulet. (2011). Characterization and Functional Assessment of Clostridium Histolyticum Class I (C1) Collagenases and the Synergistic Degradation of Native Collagen in Enzyme Mixtures Containing Class II (C2) Collagenase. Transplantation Proceedings. 43. 3171-5. https://doi.org/10.1016/j.transproceed.2011.09.059.
Successful Human Islet Isolation and Transplantation Indicating the Importance of Class 1 Collagenase and Collagen Degradation Activity Assay
Development and Application of Purified Tissue Dissociation Enzyme Mixtures for Human Hepatocyte Isolation
Roberto Gramignoli, Michael L. Green, Veysel Tahan, et al. (2012) :Development and Application of Purified Tissue Dissociation Enzyme Mixtures for Human Hepatocyte Isolation, Cell Transplantation, DOI:10.3727/096368911X600939
Effectiveness of Different Molecular Forms of C. histolyticum Class I Collagenase to Recover Islets
Michael L. Green, Andrew G. Breite, Caleb A. Beechler, Francis E. Dwulet & Robert C. McCarthy (2017): Effectiveness of different molecular forms of C. histolyticum class I collagenase to recover islets, Islets, DOI: 10.1080/19382014.2017.1365996
A New Enzyme Mixture to Increase the Yield and Transplant Rate of Autologous and Allogeneic Human Islet Products
Tissue Dissociation Enzymes for Isolating Human Islets for Transplantation: Factors to Consider in Setting Enzyme Acceptance Criteria
McCarthy RC, Breite AG, Green ML, Dwulet FE. Tissue dissociation enzymes for isolating human islets for transplantation: factors to consider in setting enzyme acceptance criteria. Transplantation. 2011;91(2):137-45.
Tissue Dissociation Enzyme Neutral Protease Assessment
Breite A, Dwulet F, McCarthy R. Tissue dissociation enzyme neutral protease assessment. Transplantation proceedings. 2010;42(6):2052-4.
Development and Characterization of a Collagen Degradation Assay to Assess Purified Collagenase Used in Islet Isolation
McCarthy RC, Spurlin B, Wright MJ, Breite AG, Sturdevant LK, Dwulet CS, et al. Development and characterization of a collagen degradation assay to assess purified collagenase used in islet isolation. Transplant Proc. 2008;40(2):339-42.
Optimizing Porcine Islet Isolation to Markedly Reduce Enzyme Consumption Without Sacrificing Islet Yield or Function
Holdcraft RW, Green ML, Breite AG, Circle L, Meyer ED, Adkins H, et al. Optimizing Porcine Islet Isolation to Markedly Reduce Enzyme Consumption Without Sacrificing Islet Yield or Function. Transplantation Direct. 9000;Online Now.