AOF BP Protease
(Cat. # 003-1000)


GMP Grade BP Protease is an affordable, aseptically dispensed, lyophilized protein containing purified neutral protease from Bacillus polymyxa.1 This 1.1 million NP Unit pack size can be used to work with our collagenases to isolate islets, hepatocytes, human epithelial skin cells, and adipose derived stromal cells.

  • Manufactured for superior lot-to-lot enzyme consistency for improved performance consistency
  • Low contaminating endotoxin levels (<50 EU/OD)
  • Lot specific Certificate of Analysis provided with complete QC analysis results
  • White lyophilized cake under vacuum in amber bottle sealed with butyl rubber stopper
  • Vial contains 1.1 million Neutral Protease (NP) units, with total NP activity >800,000 NP Units
  • Stable for at least 2 years when stored at < -20 °C

This product is purified from culture supernatants of of B. polymyxa using column chromatography. No animal derived components were used in the culture or the subsequent purification steps.

After assessing enzyme potency, the protease is aseptically dispensed into depyrogenated vials and lyophilized. When dry, the BP Protease vials are sealed under vacuum. A representative sample from the batch is reconstituted and analyzed by a variety of quality control tests. Results are compared to established specifications before release into inventory.

Suggested Assays

The specific neutral protease activity of purified BP Protease is determined by the FITC-BSA substrate NP3. The specific activity is used to provide a total target number of NP units.


  1. McCarthy RC, Dwulet FE, Breite AG, Green M. World Stem Cell Summit 2014 – San Antonio, TX “A Cost-effective, Improved Preparation of Purified Paenbacillus polymyxa Neutral Protease (Dispase® Equivalent Enzyme)”

  2. Balamurugan AN, Green M, Breite A, Loganathan G, Wilhem J, Vargova L, Lockridge A, Hering B, Dwulet F, McCarthy R. 2014 World Transplant Congress – San Francisco, CA  “Human Pancreas Digestion and Islet Yields When Using With Purified P. polymyxa Protease Enzyme Mixtures”

Find the Pack Insert, Safety Data Sheet, Specifications, and Certificate of Animal Origin in the Product Documentation page.

Note: To obtain Certificates of Analysis for our products, please contact VitaCyte. Have your product lot number readily available to help us expedite your request.