by Jessica Helweg | Nov 10, 2016
Application-specific formulations are pre-blended mixtures of purified collagenase and protease in optimal ratios for isolation of cells from a particular tissue and/or species. Currently, our CIzyme™ AS and CIzyme™ RI are our only two...
by Jessica Helweg | Nov 10, 2016
There are two components to inactivation of the digestive enzymes commonly used for tissue dissociation. To inactivate protease activity, a serum-containing media or buffer is most effective. Buffers or media containing human serum albumin (HSA) are ineffective in...
by Jessica Helweg | Nov 10, 2016
Thermolysin and BP Protease are entirely animal origin free. A porcine gelatin peptone is used during the fermentation step of natural collagenase production. While this animal sourced material is required to stimulate collagenase production from the biosynthesis from...