Application-specific formulations are pre-blended mixtures of purified collagenase and protease in optimal ratios for isolation of cells from a particular tissue and/or species. Currently, our CIzyme™ AS and CIzyme™ RI are our only two application-specific formulations.
There are two components to inactivation of the digestive enzymes commonly used for tissue dissociation. To inactivate protease activity, a serum-containing media or buffer is most effective. Buffers or media containing human serum albumin (HSA) are ineffective in suppressing protease activity, even at very high concentrations of protein (>10%). However, serum-containing buffers do not inhibit
Thermolysin and BP Protease are entirely animal origin free. A porcine gelatin peptone is used during the fermentation step of natural collagenase production. While this animal sourced material is required to stimulate collagenase production from the biosynthesis from the organism Clostridium histolyticum during anaerobic fermentation, it represents a very low adventitious agent risk as the