Collagenase Selection & Process Optimization for High Yield Hepatocyte Isolation

Thursday, May 23rd, 2024 | 11 AM ET

Presented by
Dr. Rafal P. Witek

This webinar will review best practices for building a high-yield primary human and animal hepatocyte isolation process with the inclusion of collagenase selection and buffer optimization. The webinar will cover process variabilities that impact isolation and how to overcome them to generate high-quality and high-viability hepatocytes. The information presented will enable participants to build their isolation, resulting in a high success rate and hepatocyte yield.

About the Presenter

Dr. Rafal P. Witek

Dr. Rafal P. Witek is a highly accomplished interdisciplinary scientist with over 24 years of work history in industry and academia. He is a hepatologist and expert in cellular liver and GI biology. Currently, Dr. Witek is a Senior Director of Engineering at Dimension Inx supporting R&D in 3D tissue printing and development of therapeutic treatments for various solid organ diseases, including liver.  

Prior, Dr. Witek was the Head of the Liver Program and Translational Sciences at Aspect Biosystems, Vancouver, British Columbia, and a Director of Cell Therapy Platform at Ambys Medicines in South San Francisco, CA. In both roles, he was developing CMC manufacturing processes for hepatocyte-based cell therapies.

Dr. Witek extensive career also includes a significant period at Thermo Fisher Scientific, where he served as an Associate Director of R&D, ADME/Tox. He led hepatic R&D teams, optimizing hepatocyte isolation processes, and developing various media and reagents.

Dr. Witek earned his PhD in Interdisciplinary Medical Sciences from the University of Florida in 2005 and in 2013, Dr. Witek completed an MBA in Strategy and Business Development at Duke University – The Fuqua School of Business.