Most collagenase and protease products stand on a firm foundation of decades of research and use in labs across the world. VitaCyte’s unique contributions are simple. The first is the consistency of our formulations. Our manufacturing processes give us better control over key enzyme fermentation processes, which means no need for lot testing because our products are designed and developed for superior consistency.
Second, we know the science. VitaCyte’s co-founders were key contributors on the project team that developed the first commercially-available, purified collagenase-protease enzyme blend for clinical use. Today, the VitaCyte team continues to push the boundaries of the science of cell isolation and contribute to the ongoing research, design, and development of these critical reagents and their uses.
DE Collagenase Optimization Kit: a fresh approach to defining enzyme composition and dose for maximal cell recovery
Loganathan G, Hughes MG, Szot GL, Smith KE, Hussain A, Collins DR, Green ML, Dwulet FE, Williams SK, Papas KK, McCarthy RC, Balamurugan AN. Low Cost, Enriched Collagenase-Purified Protease Enzyme Mixtures Successfully Used for Human Islet Isolation. OBM Transplantation 2019;3(2):14; doi:10.21926/obm.transplant.1902064.
Loganathan G, Subhashree V, Narayanan S, et al. Improved recovery of human islets from young donor pancreases utilizing increased protease dose to collagenase for digesting peri‐islet extracellular matrix. Am J Transplant. 2018;00:1–13. https://doi.org/10.1111/ajt.15111.
Loganathan G, Subhashree V, Breite AG, et al. Beneficial effect of recombinant rC1rC2 collagenases on human islet function: Efficacy of low‐dose enzymes on pancreas digestion and yield. Am J Transplant. 2018;18:478–485. https://doi.org/10.1111/ajt.14542.
N. Balamurugan, Appakalai & Green, Mike & Breite, Andrew & Loganathan, Gopalakrishnan & Wilhelm, Josh & Tweed, Ben & Vargova, Lenka & Lockridge, Amber & Kuriti, Manikya & Hughes, Jr, Michael & Williams, Stuart & J. Hering, Bernhard & E. Dwulet, Francis & Mccarthy, Robert. (2015). Identifying Effective Enzyme Activity Targets for Recombinant Class I and Class II Collagenase for Successful Human Islet Isolation. Transplantation Direct. 2. 1. 10.1097/TXD.0000000000000563.
A.G. Breite, R.C. McCarthy, F.E. Dwulet. (2011). Characterization and Functional Assessment of Clostridium Histolyticum Class I (C1) Collagenases and the Synergistic Degradation of Native Collagen in Enzyme Mixtures Containing Class II (C2) Collagenase. Transplantation Proceedings. 43. 3171-5. https://doi.org/10.1016/j.transproceed.2011.09.059.
Roberto Gramignoli, Michael L. Green, Veysel Tahan, et al. (2012) :Development and Application of Purified Tissue Dissociation Enzyme Mixtures for Human Hepatocyte Isolation, Cell Transplantation, DOI:10.3727/096368911X600939
Michael L. Green, Andrew G. Breite, Caleb A. Beechler, Francis E. Dwulet & Robert C. McCarthy (2017): Effectiveness of different molecular forms of C. histolyticum class I collagenase to recover islets, Islets, DOI: 10.1080/19382014.2017.1365996
McCarthy RC, Breite AG, Green ML, Dwulet FE. Tissue dissociation enzymes for isolating human islets for transplantation: factors to consider in setting enzyme acceptance criteria. Transplantation. 2011;91(2):137-45.
McCarthy RC, Spurlin B, Wright MJ, Breite AG, Sturdevant LK, Dwulet CS, et al. Development and characterization of a collagen degradation assay to assess purified collagenase used in islet isolation. Transplant Proc. 2008;40(2):339-42.
Holdcraft RW, Green ML, Breite AG, Circle L, Meyer ED, Adkins H, et al. Optimizing Porcine Islet Isolation to Markedly Reduce Enzyme Consumption Without Sacrificing Islet Yield or Function. Transplantation Direct. 9000;Online Now.
Stull, Natalie & Breite, Andrew & Mccarthy, Robert & Tersey, Sarah & Mirmira, Raghavendra. (2012). Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease. Journal of visualized experiments : JoVE. 10.3791/4137.
Bashar Khiatah, Amber Tucker, Kuan-Tsen Chen, Rachel Perez, Shiela Bilbao, Luis Valiente, Leonard Medrano, Jeffrey Rawson, Elena Forouhar, Keiko Omori, Fouad Kandeel, Meirigeng Qi & Ismail H. Al-Abdullah (2018): Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata, Islets, DOI: 10.1080/19382014.2017.1417716.
Green M, Beechler C, Breite A, Dwulet F, McCarthy R. 12th Congress of the International Xenotransplantation Society 2013 – Osaka, Japan #250 “Optimization of a Porcine Islet Isolation and Purification Procedure that Utilizes Recombinant Collagenase”
Breite AG, Dwulet FE, McCarthy RC. 13th World Congress of IPITA 2011 – Prague, Czech Republic O-038 “Characterization and functional assessment of Clostridium histolyticum class I (C1) collagenases and the synergistic degradation of native collagen in enzyme mixtures containing class II (C2) collagenase”
McCarthy RC, Breite AG, Dwulet FE. 8th Annual IFATS Conference 2010 – Dallas, TX “Biochemical Analysis of Crude Collagenase Products Used in Adipose Derived Stromal Cell (ADSC) Isolation Procedures and Development of a Purified Tissue Dissociation Enzyme (TDE) Mixture”
Breite AG, McCarthy RC, Dwulet FE. International Pancreas and Islet Transplantation Association – International Xenotransplantation Association Joint Meeting 2009 – Venice, Italy Presentation IPITA O 3.7 “Assessing Neutral Protease Activity in Tissue Dissociation Enzyme Mixtures”
McCarthy RC, Breite AG, et al. MSC Regenerative Medicine and Adult Stem Cell Therapy 2009 – Cleveland, OH “Analysis of the Enzyme Composition of Crude Collagenase Products Used in Adipose Derived Stromal Cell Isolation Procedures”