Publications & Presentations

Most collagenase and protease products stand on a firm foundation of decades of research and use in labs across the world. VitaCyte’s unique contributions are simple. The first is the consistency of our formulations. Our manufacturing processes give us better control over key enzyme fermentation processes, which means no need for lot testing because our products are designed and developed for superior consistency.

Second, we know the science. VitaCyte’s co-founders were key contributors on the project team that developed the first commercially-available, purified collagenase-protease enzyme blend for clinical use. Today, the VitaCyte team continues to push the boundaries of the science of cell isolation and contribute to the ongoing research, design, and development of these critical reagents and their uses.


White Papers

Lot Qualification: A Common but Costly Pathway to Select Collagenase Products for Cell Isolation

Overcoming the Limitations of Traditional Collagenase Products

DE Collagenase Optimization Kit: A Fresh Approach to Defining Enzyme Composition and Dose for Maximal Cell Recovery

What is an Acceptable Rodent Islet Yield?



Evolution of Enzyme Requirements for Human Islet Isolation


Improved Recovery of Human Islets From Young Donor Pancreases Utilizing Increased Protease Dose to Collagenase for Digesting Peri‐Islet Extracellular Matrix


Beneficial Effect of Recombinant rC1rC2 Collagenases on Human Islet Function: Efficacy of Low‐Dose Enzymes on Pancreas Digestion and Yield


Identifying Effective Enzyme Activity Targets for Recombinant Class I and Class II Collagenase for Successful Human Islet Isolation


Characterization and Functional Assessment of Clostridium Histolyticum Class I (C1) Collagenases and the Synergistic Degradation of Native Collagen in Enzyme Mixtures Containing Class II (C2) Collagenase


Successful Human Islet Isolation and Transplantation Indicating the Importance of Class 1 Collagenase and Collagen Degradation Activity Assay


Development and Application of Purified Tissue Dissociation Enzyme Mixtures for Human Hepatocyte Isolation


Effectiveness of Different Molecular Forms of C. histolyticum Class I Collagenase to Recover Islets


A New Enzyme Mixture to Increase the Yield and Transplant Rate of Autologous and Allogeneic Human Islet Products


Tissue Dissociation Enzymes for Isolating Human Islets for Transplantation: Factors to Consider in Setting Enzyme Acceptance Criteria


Tissue Dissociation Enzyme Neutral Protease Assessment


Development and Characterization of a Collagen Degradation Assay to Assess Purified Collagenase Used in Islet Isolation


Optimizing Porcine Islet Isolation to Markedly Reduce Enzyme Consumption Without Sacrificing Islet Yield or Function


Mouse Islet of Langerhans Isolation Using a Combination of Purified Collagenase and Neutral Protease



Green M, Beechler C, Breite A, Dwulet F, McCarthy R. 12th Congress of the International Xenotransplantation Society 2013 – Osaka, Japan #250 “Optimization of a Porcine Islet Isolation and Purification Procedure that Utilizes Recombinant Collagenase”

Breite AG, Dwulet FE, McCarthy RC. 13th World Congress of IPITA 2011 – Prague, Czech Republic O-038 “Characterization and functional assessment of Clostridium histolyticum class I (C1) collagenases and the synergistic degradation of native collagen in enzyme mixtures containing class II (C2) collagenase”

McCarthy RC, Breite AG, Dwulet FE. 8th Annual IFATS Conference 2010 – Dallas, TX “Biochemical Analysis of Crude Collagenase Products Used in Adipose Derived Stromal Cell (ADSC) Isolation Procedures and Development of a Purified Tissue Dissociation Enzyme (TDE) Mixture”

Breite AG, McCarthy RC, Dwulet FE. International Pancreas and Islet Transplantation Association – International Xenotransplantation Association Joint Meeting 2009 – Venice, Italy  Presentation IPITA O 3.7 “Assessing Neutral Protease Activity in Tissue Dissociation Enzyme Mixtures”

McCarthy RC, Breite AG, et al. MSC Regenerative Medicine and Adult Stem Cell Therapy 2009 – Cleveland, OH “Analysis of the Enzyme Composition of Crude Collagenase Products Used in Adipose Derived Stromal Cell Isolation Procedures”