VitaCyte offers purified collagenase and neutral protease products for tissue dissociation on a variety of tissue types. Unlike other collagenase products on the market, CIzyme™ collagenases are offered at three different activity levels for applications on different tissue types. This novel approach to offering collagenase enzymes reflects the knowledge from improved biochemical assay to understand C. histolyticum collagenase specific collagen degrading activities. Crude collagenases (Worthington I, Sigma IV, etc.) are purified and the components analyzed by a proprietary purification protocol and an improved collagen-degrading assay (CDA) to quantitate each component. The rationale for offering collagenase at different activity levels is the ability to match traditional crude collagenases that work well with certain cell or tissue types using purified components. This will ensure greater reproducibility and benefit from the low endotoxin levels and insoluble components common in most crude collagenases.
CIzyme™ collagenase and neutral protease enzymes are packaged separately to maintain the greatest long-term stability. Rigorous stability data assessments have shown the products to be stable as a lyophilized powder for 2 years when maintained as individual components. A series of experiments performed on the reconstituted enzymes show that when the collagenase and neutral protease enzymes are frozen separately they are able to withstand several freeze-thaw cycles with no apparent loss of activity. The only exception is CIzyme™ BP Protease which is sensitive to freeze-thaw cycles when in solution. Users that require a sterile solution can filter the blended enzymes with standard hydrophilic low protein binding filters such as cellulose acetate and PES with no apparent loss of activity. Details about these experiments can be found in the appropriate pack inserts.