Tissue Dissociation Enzyme (TDE) Publications

Enzymes

• Collagenase

• Harper E. Collagenases. Annu Rev Biochem. 1980; 49:1063-78. Online Reference

• Mookhtiar KA and Van Wart HE. Clostridium histolyticum collagenases: a new look at some old enzymes. Matrix Supplement 1992; 1:116-26 Online Reference

• Matsushita O and Okabe A. Clostridial hydrolytic enzymes degrading extracellular components. Toxicon. 2001; 39:1769-80 Online Reference

• Thermolysin

• Matsubara H. Methods in Enzymology 1970; 19:642-50.

• BP Protease

• Griffin PJ and Fogarty WH. Physiochemical Properties of the Native, Zinc-and Manganese-Prepared Metalloprotease of Bacillus polymyxa. Applied Microbiol. 1973; 26(2):191-195. Online Reference

• Fogarty WH and Griffin PJ. Production and Purification of the Metalloprotease of Bacillus polymyxa. Applied Microbiol. 1973; 26(2):185-190. Online Reference

• Clostripain

• Mitchell WM and Harrington WF. Methods in Enzymology 1970; 19:635-42.

Assay Publications

• Collagenase Assays (by substrate)

• Collagen degradation assay using collagen fibers: Mandl I, Zipper H, Ferguson LT. Clostridium histolyticum collagenase: its purification and properties. Arch. Biochem. Biophys. 1958; 74:465-475. Online Reference

• Collagen degradation assay using FITC collagen fibrils: McCarthy RC, Spurlin B, Wright MJ, Breite AG, Sturdevant LK, Dwulet CS, Dwulet FE. Transplant Proc. 2008; 40(2):339-42. Online Reference

• Pz peptide: Wunsch E, Heidrich HC. Zur quantitativen Bestimmung der Kollagenase. Z. Physiol. Chem. 1963; 333:149.

• FALGPA peptide: Van Wart HE and Steinbrink DR. Analytical Biochemistry. 1981; 113(2):356-65. Online Reference

• Neutral Proteases Assays (by substrate)

• Dimethyl casein: Lin Y, et al. The Action of Proteolytic Enzymes on N,N-dimethyl Proteins. Basis for a Microassay for Proteolytic Enzymes. J. Biol. Chem. 1969; 244:789-793. Online Reference

• FITC bovine serum albumin: Voss EW, Workman CJ, and Mummert ME. Detection of Protease Activity using a Fluorescence-Enhancement Globular Substrate. Biotechniques 1996; 20:286-91. Online Reference

• FITC casein: Twining SS. Fluorescein Isothiocyanate-labeled Casein Assay for Proteolytic Enzymes. Analytical Biochemistry 1984; 143:30-4. Online Reference

• BAEE for trypsin like activity: Mitchell WM and Harrington WF. Methods in Enzymology 1970; 19:635-42.