The cell yield and quality of isolated human hepatocytes depends on the quality of liver tissue and the composition and concentration of the tissue dissociation enzymes (TDEs) used in the cell isolation procedure. This post reviews data on VitaCyte’s recommended purified TDE composition specifically designed to isolate human hepatocytes. The hepatocyte isolation procedures were performed in Doctor Stephen Strom’s laboratory at the University of Pittsburgh. The rigorous definition of the purity and activity of the enzymes used in this product overcomes many of the problems that are encountered when using crude or enriched collagenase in human hepatocyte isolation.
Archive for December, 2009
Purified tissue dissociation enzymes for human hepatocyte isolation
Wednesday, December 2nd, 2009Application of purified tissue dissociation enzymes for isolation of rodent hepatocyte isolation
Wednesday, December 2nd, 2009
A review of selected literature below summarizes the concentration of purified tissue dissociation enzymes used to isolate mouse or rat (i.e., rodent) hepatocytes. The two major advantages of using purified enzymes in place of crude or enriched collagenase products is the consistency of manufacture and minimal endotoxin contamination. The data presented below shows the range of purified enzyme concentrations used in modifications to Seglen’s original procedure to isolate rodent hepatocytes1 and presents a recommendation for using VitaCyte’s CIzyme purified enzyme products in this application.
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