The introduction of Liberase™ HI Purified Enzyme Blend (HI) in November, 1994 was the first time a purified tissue dissociation enzyme product was developed to isolate human islets. This product contained purified collagenase (class I and class II) from Clostridium histolyticum and a purified bacterial neutral protease (thermolysin or Dispase®). The introduction of Liberase HI was followed by a number of similar Liberase™ purified products with different concentrations of collagenase and neutral protease. At this time, there is limited availability of these products. The information and table below provides a translation of Roche’s Liberase products to VitaCyte’s CIzyme™ purified tissue dissociation enzyme products.
Archive for April, 2009
Comparison of VitaCyte’s purified tissue dissociation enzymes with competitive products.
Thursday, April 16th, 2009VitaCyte facilitates collaboration within the cell isolation community.
Wednesday, April 15th, 2009VitaCyte is committed to improving the reagents, methods/processes and cells isolated from mammalian tissues. We fulfill this commitment by maintaining an active research program that allows us to ask fundamental questions about the effectiveness of collagenase and other proteases to release cells from tissue. Enzymatic tissue dissociation is a complex process that requires collaboration among the members of the cell isolation community. To this end, VitaCyte’s website uses blog software, allowing us to share our understanding of tissue dissociation enzymes with customers of tissue dissociation enzyme products. The goal of this effort is to stimulate a productive exchange of knowledge and ideas that will lead to improvements in cell isolation processes leading to more effective utilization of research funding. If you have any ideas or comments that will accelerate progress toward this goal, please feel free to contact me at any time at feedback@vitacyte.com.
Bob McCarthy
President, VitaCyte LLC
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